The pharmacokinetics of cefquinome was studied following intravenous and intramuscular (single & repeated) injection . Cefquinome was assayed by High performance liquid chromatography method Following a single intravenous injection of 2 mg/kg body weight of cefquinome in normal chickens, plasma concentration - time curve was best described by a three compartments model with elimination half - life (t 0.5(β) = 0.712 hour), volume of distribution (V dss =389.23 ml/kg) and total clearance of the drug (C l tot = 0.048 ml/kg/min). Following a single intramuscular administration of 2 mg/kg body weight cefquinome in normal chickens, the peak plasma concentration ( C max ) was 3.53 μg/ml was achieved at a maximum time (T max ) of 2.80 hour. The mean systemic bioavailability was 106.82%. The plasma concentrations of cefquinome following repeated intramuscular administration of 2 mg/kg body weight once daily for three cons ecutive days in normal and experimentally S almonella e ntretidis infected chickens showed a lower significant values recorded in experimentally Salmonella e ntretidis infected chickens than in normal ones. Cefquinome showed accumulative behavior in plasma of chickens. Cefquinome was assayed in plasma, heart, liver, lung, kidney, breast muscle, thigh muscle and skin after 24, 48, 72, 96, 120 and144 hours post last dose following administration of 2 mg/kg body weight every 24 hours. Results of this study indica ted that cefquinome was useful for treatment of Salmonella e ntretidis infections in chickens.