Staff Members - Benha University
Evaluation of DNA fragmentation in teratozoospermic infertile men compared with normozoospermic fertile men and its correlation with sperm morphology
• 2015
Publication Information
Authors
Yasmin Magdia, Ehab Darwisha AQ2 , Salah Elbashirb and Mona Ahmed Elawady
Keywords
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Journal
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Publisher
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Volume
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Issue
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Pages
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publication.type
International
Paper Link
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Supplementary Materials
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Abstract
Purpose
This study aimed to examine the relationship between sperm morphology and sperm
DNA fragmentation in infertile teratozoospermic men and fertile normozoospermic
men.
Materials and methods
Semen samples were collected from 69 patients classified as infertile
teratozoospermic men and 65 healthy fertile normozoospermic men (control). Semen
parameters were assessed using WHO 2010 guidelines. Spermatozoa DNA integrity
was assessed using the method of the sperm chromatin dispersion test.
Results
Sperm DNA fragmentation was statistically higher in infertile teratozoospermic men
compared with fertile men (P =0.001) and the percentage of fragmented sperm had
significant positive correlations with morphologically abnormal sperm (P =0.001).
Conclusion
On the basis of these findings, it is important to assess sperm DNA damage in the
diagnosis of male infertility. Our data also showed a negative correlation between the
degree of DNA fragmentation and the morphology of sperm in semen samples
This study aimed to examine the relationship between sperm morphology and sperm
DNA fragmentation in infertile teratozoospermic men and fertile normozoospermic
men.
Materials and methods
Semen samples were collected from 69 patients classified as infertile
teratozoospermic men and 65 healthy fertile normozoospermic men (control). Semen
parameters were assessed using WHO 2010 guidelines. Spermatozoa DNA integrity
was assessed using the method of the sperm chromatin dispersion test.
Results
Sperm DNA fragmentation was statistically higher in infertile teratozoospermic men
compared with fertile men (P =0.001) and the percentage of fragmented sperm had
significant positive correlations with morphologically abnormal sperm (P =0.001).
Conclusion
On the basis of these findings, it is important to assess sperm DNA damage in the
diagnosis of male infertility. Our data also showed a negative correlation between the
degree of DNA fragmentation and the morphology of sperm in semen samples