Theme-Logo
  • Login
  • Home
  • Course
  • Publication
  • Theses
  • Reports
  • Published books
  • Workshops / Conferences
  • Supervised PhD
  • Supervised MSc
  • Supervised projects
  • Education
  • Language skills
  • Positions
  • Memberships and awards
  • Committees
  • Experience
  • Scientific activites
  • In links
  • Outgoinglinks
  • News
  • Gallery
publication name Axenic in vitro culture of Schistosoma mansoni miracidia.
Authors Amr A. Rashed, Kamal K .Shehata, Brain L. James and Mona E. Nasr
year 1996
keywords
journal
volume Not Available
issue Not Available
pages Not Available
publisher Not Available
Local/International International
Paper Link Not Available
Full paper download
Supplementary materials Not Available
Abstract

Miracidia of Schistosoma monsoni have been grown axenically. The use of saline solution of 0.75% facilitated the shedding of epidermal plates which if occurred promote a rapid sporocystogenesis. Also, the use of a ratio of 1:3 (fetal calf serum and medium 199) in addition to chernin balanced salt solution (SBSS) was ideal for maintaining sporocysts up to 14 days. All the miracidia sheded their epidermal plates 2 hours post cultivation. At the end of the second week post cultivation, the daughter sporocysts measured 690u in length by 50u in width. The usage of plastic flat sided flat sided flasks with plenty of culture medium was very important for induction a proper growth of sporocysts to each other might delay their growth.

Benha University © 2023 Designed and developed by portal team - Benha University