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DNA riboprinting analysis of Tilapia species and their hybrids using restriction fragment length polymorphisms of the small subunit ribosomal DNA
Aquaculture Resaerch • 2007
Publication Information
Authors
Sabry S El-Serafy, Nassr-Allah H Abdel-Hameid, Mohammed H Awwad & Mona S Azab
Keywords
tilapia, 18S srDNA, RFLP, morphometric,
meristic, hybrids
Journal
Aquaculture Resaerch
Publisher
wiley
Volume
38
Issue
Not Available
Pages
295-303
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Morphometric, meristic and DNA riboprinting analyses
of Tilapia species and their hybrids inhabiting
the River Nile were examined. Morphometric data
showed striking similarities and overlapping among
Tilapia species, making it impossible to di¡erentiate
these species. Meristic characteristics revealed that
Tilapia species could be identi¢ed into four major
groups (Oreochromis niloticus, O. aureus, Sarotherodon
galilaeus and Tilapia zillii). The lateral line scales differed
signi¢cantly between the four Tilapia species,
while the number of ¢n rays in the dorsal and anal
¢ns di¡ered signi¢cantly, di¡erentiating three species
(but not between O. niloticus and O. aureus). Restriction
fragment length polymorphisms (RFLPs) of nuclear
small sub-unit ribosomal RNA (18S srRNA)
gene were used to di¡erentiate the species. Polymerase
chain reaction-restriction fragment length polymorphisms
data provided a unique pattern for each
species with a speci¢c restriction enzyme. Two hybrids
of Tilapia designated H1 and H2 were detected.
The endonucleases SacII and ApaI di¡erentiated H1
and H2. This research revealed a monophylogenetic
relationship among all the studiedTilapia species.
of Tilapia species and their hybrids inhabiting
the River Nile were examined. Morphometric data
showed striking similarities and overlapping among
Tilapia species, making it impossible to di¡erentiate
these species. Meristic characteristics revealed that
Tilapia species could be identi¢ed into four major
groups (Oreochromis niloticus, O. aureus, Sarotherodon
galilaeus and Tilapia zillii). The lateral line scales differed
signi¢cantly between the four Tilapia species,
while the number of ¢n rays in the dorsal and anal
¢ns di¡ered signi¢cantly, di¡erentiating three species
(but not between O. niloticus and O. aureus). Restriction
fragment length polymorphisms (RFLPs) of nuclear
small sub-unit ribosomal RNA (18S srRNA)
gene were used to di¡erentiate the species. Polymerase
chain reaction-restriction fragment length polymorphisms
data provided a unique pattern for each
species with a speci¢c restriction enzyme. Two hybrids
of Tilapia designated H1 and H2 were detected.
The endonucleases SacII and ApaI di¡erentiated H1
and H2. This research revealed a monophylogenetic
relationship among all the studiedTilapia species.