Glycerol is used for treating brain edema .The potential toxicity increases in glycerol kinase deficiency is associated with glycerol levels in plasma and urine that are more than 100-fold higher than normal . The present study aims to investigate the protective effects of curcumin on glycerol induced acute renal failure using a model of glycerol intra-peritoneal administration with 24 h dehydration in albino rats. Twenty-four hours after the administration of glycerol, all rats were sacrificed. Urea and creatinine levels were measured in the blood, and the levels of malondialdehyde, superoxide dismutase and glutathione-S- transferase activity in renal homogenate were determined in the renal tissue. Glycerol administration resulted in elevation in levels of renal malondialdehyde in addition to reduction of superoxide dismutase and glutathione-S- transferase activity and deterioration in the renal functions as assesseted by the increased in plasma urea and creatinine as compared to control rats. Curcumin in a dose of 200-mg/kg body weights for 30 min after the injection of glycerol significantly reduced the elevated malondialdehyde levels and increased the antioxidant enzyme activity in rats treated with intra-peritoneal glycerol. In conclusion curcumin might be a potential candidate agent against glycerol-induced nephrotoxicity.