Query (Q) fever is an ubiquitous zoonosis caused by Coxiella burnetii . The present study was carried out to determine the prevalence of C. b urnetii in apparent ly healthy sheep, goats and farm workers. Raw milk and serum samples were randomly collected from 200 sheep, goat s (100 of each) and 30 farm workers from Qaluobia governorate, Egypt during 2014/2015. The milk and serum samples were inves tigated for IgG antibodies against C. burnetii phase II antigen by indirect immunofluorescent antibody test (IFAT). The seropositive samples were confirmed by touch – down PCR with specific primers which amplify transposon -like region of C. burnetti . The re sults showed that antibodies against C. burnetii in sheep raw milk and sera were 17% and 23% respectively, in goat raw milk and sera were 19% and 27% respectively and in human was 23.3% . PCR targeting IS 1111 gene confirmed the presence of C. burnetti DNA in sheep and goats raw milk and sera were 82.4%, 89.5%, 91.3% and 85.2% respectively and in farm workers was 57.1% . The se results proved that the apparently healthy sheep and goats are an important reservoir of C.burnetii infection . The farm workers cons titute an occupational risk group for C. burnetii infection, for their contact with infected livestock