PURPOSE: Gastric cancer (GC) is an extremely common disease worldwide. The p16 INK4a protein, is a tumor suppressor protein that inhibits CDK4 and CDK6, which phosphorylate the RB protein. S100A4 is known to be involved in cancer cell motility by virtue of its ability to activate non-muscle myosin. The present study aims at investigating role of p16 INK4a, and S100A4 in progression of gastric carcinoma by analyzing p16 INK4a and S100A4 protein expression in gastric carcinomas and correlating their expression with clinicopathological findings.
Patients and Methods: Forty cases including 30 non-consecutive retrospective selected gastric carcinomas (4 GI, 16 GII, 10 GIII), and 10 cases of normal gastric tissue at resection margins of peptic ulcers, were taken as control. Cases were collected in the period 2009-2012, selected from files of pathology department, Faculty of Medicine- Benha University and Egyptian National Cancer Institute (NCI). Follow up of the selected cases was recorded for 18 months. Correlations between S100A4 and p16 INK4a immunoreactivity and clinicopathological characteristics were evaluated.
Results: showed significant inverse correlation between p16 INK4a expression and grade of carcinoma (P < 0.05) as well as high significant correlation with type of gastric carcinoma (P < 0.01). S100A4 was positively correlated with tumor grade, lymph node metastasis, distant metastasis and tumor-node-metastasis (TNM) staging (P