Background: The changes in the gastric environment that occur because of Helicobacter Pylori (H. pylori) infection are complicated . The combination of these changes and gastric microbiota could lead to progression of many gastric disorders. Objective: This study aimed to assay the gastric bacterial microbiota in patients infected with H. pylori with dyspeptic symptoms and without. Patients and Methods: This study included 70 naive infected H. pylori patients .Cases were divided into two equal groups, Group 1: patients with chronic dyspeptic symptoms 3 months ago diagnosed by Rome IV criteria . Group 2: patients without dyspeptic symptoms. Upper esophagogastroduodenoscopy done for all participants and gastric biopsies were taken for bacterial cultures that were assayed by Vitek 2 system to identify colonies of gastric microbiota for all patients. Results: isolated gram-negative organisms were higher in non-dyspeptic than dyspeptic group (P = 0.015). Isolated Staphylococci were significantly higher in dyspeptic group compared to non-dyspeptic group (P = 0.003). Streptococci and Klebsiella were significantly decreased in dyspeptic group than non-dyspeptic group (P = 0.019 and 0.001 respectively) but other genus were insignificantly different between both groups. Conclusion : Gastric microbiota could affect the appearance of dyspepsia in H. pylori infected patients.