Background and study aim: Hepatitis B is a serious and common infectious disease of the liver, affecting millions of people throughout the world. In this regard, Egypt has an intermediate HBV seroprevalence. Quantitation of hepatitis B surface antigen (HBsAg) by automated chemiluminescent micro-particle immunoassay has been proposed to be a surrogate marker. The aim of this study was to evaluate correlation between HBsAg quantitation assay, histopathology and viral-DNA level in chronic hepatitis HBeAg negative patients. Patients and methods: This study enrolled in the study 50 naive patients with Chronic hepatitis B virus, consecutively observed at Hepatology department at Mahalla Teaching Hospital in the period between May 2013 to October 2014. All the studied patients were HBeAg negative. HBV DNA was measured by real-time polymerase chain reaction, and serum HBsAg was quantified by electrochemiluminescence assay (Roche Diagnostic). Liver biopsy was done to all patients in this study for histopathological grading and staging of hepatic fibrosis by experienced pathologist using Metavir scoring. All patients were divided into two groups (A&B): Group A: Including 25 patients with chronic hepatitis B virus subdivided into two sub groups: Group B: Including 25 patients with chronic hepatitis B virus Subdivided into two sub groups: Results: There is no statistically significant difference between the two studied groups (