Staff Members - Benha University
Biomass colonization and bioconversion of the molecular characterized Oxalobacter formigenes to mitigate calcium oxlate urolithiasis
Biomass Conversion and Biorefinery • 2022
Publication Information
Authors
Mohamed Donia, Mohamed Zeineldin, Naglaa Gomaa, Midhat Nassif, Yamen Hegazy, Jose Cedillo Monroy, Edson Brodeli Figueroa Pacheco, Raymundo Rene Rivas-Caceres, Rabiha Seboussi, Mohamed Abdelmegeid
Keywords
Not Available
Journal
Biomass Conversion and Biorefinery
Publisher
Not Available
Volume
Not Available
Issue
Not Available
Pages
Not Available
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Calcium oxalate (CaOx) is one of the common causes of kidney stones and accounts for 40 to 50% of all uroliths in cats. Oxalobacter formigenes, an oxalate-degrading intestinal microbiota, has been hypothesized to play a protective role against CaOx urolithiasis due to its capability to degrade oxalate. This study was designed to reveal the association between biomass colonization of O. formigenes and clinical occurrence of CaOx urolithiasis in household tomcats. Fifteen tomcats were allocated into three groups (healthy control (n = 5), static chronic kidney disease (static CKD) (n = 4), and progressive CKD (n = 6)) based on diagnosis of CaOx urolithiasis and disease progression. Fecal samples were collected from all tomcats, genomic DNA was extracted, and oxc, a gene specific for O. formigenes, was quantified using real-time PCR. Additionally, the clinical association between blood serum urea …