Vitamins are important molecules that play critical roles in biochemical reactions as Vit. A and E. Vitamin A plays a role in body construction and its deficiency reflects the healthy state of body. Its active form named retinoic acid (carboxylic acid form). Vitamin E has 8 isoforms and is naturally present in eight isoforms. The most isoform known is -tocopherol. Several studies performed in humans have reported a beneficial effect of vitamin A and E on improving insulin sensitivity. Vitamin effects occur without modulation of insulin secretion in insulin resistance and atherosclerosis. In this study we tested the effect of vitamin A and E on biochemical changes of liver, kidney metabolites in obese Wistar rats. The results showed that oral administration of Vit. A and E reduced the changes in body weight and food intake. Moreover, vitamin A and E induced significant changes in GPT and Urea levels as they increased in obese rats compared to normal fed rats and normalized by vitamin A and E for urea. Finally, changes in antioxidant parameters including catalase, MDA and nitrous oxide. In conclusion, vitamin A and E ameliorated the biochemical alterations accompanied obesity in Wistar rats. Key words: Antioxidant effects; Lipid profiles; Obesity; Vit. A& E; Wistar rats