Chlorella vulgaris species was isolated from Nile River in Qalubia Governorates, Egypt. The highest constant of growth (μ=1.5), and the lower time of reproducing a generation (G=0. 2). The green alga was identified using 18S rRNA gene amplification followed by sequencing the resultant sequence was compared with those available on the NCBI website database through the BLAST bioinformatics tool. The percentage of lipids synthesized was 20 %. In order to increase lipid contents in this green alga was made chemical mutation by EMS. Using three concentrations from EMS (0.1, 0.01 and 0.001) for two hours. The resulted in the treatment of concentration 0.1 decrease total lipid to 16.2 % in by concentration 0.01 almost no change the total lipids were equal with wild strain. By the concentration 0.001 a significant increase in total lipids content as it reached 25% compared with wild strain.