The ability of microorganism to adapt the changes in the osmolality of the external milieu is of fundamental importance for growth and survival, and thus prokaryotic cells have evolved a number of osmoadaptative mechanisms to cope with elevated osmolality. One hundred bacterial isolates were isolated from soil from Egypt and screened for salt tolerance. The best four isolates were identified by manual and API 20E system methods. Identification of bacterial isolates showed that strains were Pseudomonas mallei, Halococcus salifodinae, Pseudomonas flourecens, Pseudomonas aeruginosa. The primers were designed for screening the bacterial isolates about the EctC gene from Ectoine operon. The positive result was with bacterial isolate Pseudomonas mallei. The length of observed fragment was between 300-400 bp. SDS-PAGE analysis of the four isolates revealed that the protein profile of the six isolates were different from each other in their banding pattern at different concentrations of NaCl (0.0 ppm,40.000 ppm and 58.500 ppm) respectively.