Root-knot nematodes are major pests of field and vegetable crops in Egypt and worldwide. Cause damage to many economically important horticultural crops like potato, cotton and tomato. In the 1940s the root-knot nematode resistance gene (Mi) was introgressed into the cultivated tomato from the wild species Lycopersicon peruvianum. Today, many commercial tomato varieties carry the Mi gene, which has been mapped. This gene confers resistance to Meloidogyne incognita, M. javanica and M. arenaria. The short arm of chromosome 6 and many markers linked to Mi have also been identified. The Mi gene has been isolated, cloned and sequenced. In this study, plants were infected with M. incognita race 2 for determined genotypes of resistant and susceptible. According to nematode resistance assays, the root-gall index was determined as > 2 and ≤ 2 for susceptible and resistant F1, F2 and BC1 plants, and reproduction factors were 0 and > 1 for resistant and susceptible F1, F2 and BC1 plants, respectively. In conjunction with traditional screening Mi gene specific primers were used to differentiate between resistant and susceptible plants with a1.5 to 1.6 kb DNA band being detected in resistant plants but absent in susceptible plants. The data showed a clear correlation between traditional screening and the use of markers and support the possibilities of using marker assisted selection for M. incognita resistance breeding.