Ear infections (particularly, otitis media) is a prevailing and common infection in developing countries causing local damage and threatening complications. Pseudomonas aeruginosa is the most common pathogen causing chronic suppurative otitis media (CSOM) and malignant otitis externa. The objective of this study is to identify incidence of Pseudomonas aeruginosa involved in ear infections and associated biochemical parameters which may be changed. External auditory canal in rabbit ears was inoculated with (106 ) colony-forming units (CFU) of Pseudomonas aeruginosa or left as sterile controls for eight weeks. There are significantly decrease results at (P