Staff Members - Benha University
Evaluation of nano-gold beads based-ELISA for detection of Cryptosporidium antigen in stool samples of diarrheic patients
• 2018
Publication Information
Authors
Mona E. Naser1, M.D., Mohamed S. younis1, M.D., Ibrahim R.Bayoumi2,M.D., Maysa A. Eraky1,M..D,Nagwa S. Aly,1 M.D., and Rabab E. Omar1,M.S.C
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publication.type
International
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Abstract
Background : Biomedical nanotechnology is providing opportunities for rapid and simple diagnosis of many infectious diseases.
Purpose:The aim of this study was to evaluate nano-gold beads based-ELISA for detection of Cryptosporidium antigen in stool samples of diarrheic patients.
Methods:Two hundred stool samples were collected from diarrheic patients attending Abo Elresh hospital. Parasitological examination of the collected samples by : Direct Smear, Concentration method and staining technique.Immunological examination bysandwich ELISA and nano-sandwich ELISA. Among the whole 200 diarrheic cases included in this study.
Results: There were 5stool samples positivefor Cryptosporidium detected by M.I.F(2.5%). There were47stool samples positivefor Cryptosporidium detected by M.Z.N (23.5%). There were 78 stool samples positive for Cryptosporidium detected by sandwich ELISA (39%)and 89 stool samples positive for Cryptosporidium detected by nano sandwich ELISA(44%). The sensitivity of sandwich ELISA and Nano-ELISA for detection of Cryptosporidium antigen in human stool samples were 68% and 85% respectively. The specificity of Sandwich ELISA was 77.3%but the specificityof nano-ELISA was73.4%.
Conculsion: The nanosandwich ELISA improve the sensitivity and specificity of sandwich ELISA. .
Purpose:The aim of this study was to evaluate nano-gold beads based-ELISA for detection of Cryptosporidium antigen in stool samples of diarrheic patients.
Methods:Two hundred stool samples were collected from diarrheic patients attending Abo Elresh hospital. Parasitological examination of the collected samples by : Direct Smear, Concentration method and staining technique.Immunological examination bysandwich ELISA and nano-sandwich ELISA. Among the whole 200 diarrheic cases included in this study.
Results: There were 5stool samples positivefor Cryptosporidium detected by M.I.F(2.5%). There were47stool samples positivefor Cryptosporidium detected by M.Z.N (23.5%). There were 78 stool samples positive for Cryptosporidium detected by sandwich ELISA (39%)and 89 stool samples positive for Cryptosporidium detected by nano sandwich ELISA(44%). The sensitivity of sandwich ELISA and Nano-ELISA for detection of Cryptosporidium antigen in human stool samples were 68% and 85% respectively. The specificity of Sandwich ELISA was 77.3%but the specificityof nano-ELISA was73.4%.
Conculsion: The nanosandwich ELISA improve the sensitivity and specificity of sandwich ELISA. .