| publication name | Evaluation of nano-gold beads based-ELISA for detection of Cryptosporidium antigen in stool samples of diarrheic patients |
|---|---|
| Authors | Mona E. Naser1, M.D., Mohamed S. younis1, M.D., Ibrahim R.Bayoumi2,M.D., Maysa A. Eraky1,M..D,Nagwa S. Aly,1 M.D., and Rabab E. Omar1,M.S.C |
| year | 2018 |
| keywords | |
| journal | |
| volume | Not Available |
| issue | Not Available |
| pages | Not Available |
| publisher | Not Available |
| Local/International | International |
| Paper Link | Not Available |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
Background : Biomedical nanotechnology is providing opportunities for rapid and simple diagnosis of many infectious diseases. Purpose:The aim of this study was to evaluate nano-gold beads based-ELISA for detection of Cryptosporidium antigen in stool samples of diarrheic patients. Methods:Two hundred stool samples were collected from diarrheic patients attending Abo Elresh hospital. Parasitological examination of the collected samples by : Direct Smear, Concentration method and staining technique.Immunological examination bysandwich ELISA and nano-sandwich ELISA. Among the whole 200 diarrheic cases included in this study. Results: There were 5stool samples positivefor Cryptosporidium detected by M.I.F(2.5%). There were47stool samples positivefor Cryptosporidium detected by M.Z.N (23.5%). There were 78 stool samples positive for Cryptosporidium detected by sandwich ELISA (39%)and 89 stool samples positive for Cryptosporidium detected by nano sandwich ELISA(44%). The sensitivity of sandwich ELISA and Nano-ELISA for detection of Cryptosporidium antigen in human stool samples were 68% and 85% respectively. The specificity of Sandwich ELISA was 77.3%but the specificityof nano-ELISA was73.4%. Conculsion: The nanosandwich ELISA improve the sensitivity and specificity of sandwich ELISA. .