Objective: To evaluate the effects of reduced glutathione on the quality of cryopreserved Boer
buck spermatozoa. Methods: The current study was carried out on five Boer bucks from which
semen samples were collected by artificial vagina. After microscopical evaluation at 37 曟,
semen samples that fulfill the ideal requirements for extension were diluted in a tris–based
extender including different concentrations of reduced glutathione (2, 5, 7 and 10 mM) and
those without glutathione served as a control. Sperm motility, viability, acrosome integrity,
DNA integrity, total antioxidant capacity and lipid peroxidation were assessed post-thawing.
Results: The current results revealed that post-thawing motility, viability and acrosomal
integrity were significantly improved [(66.67±5.50)%, 168.30±18.59 and (12.75±2.45)%,
respectively] when 5 mM glutathione was added to semen extender; especially as compared
with the control [(40.00 ±2.88)%, 95.00±8.90 and (25.75±3.46)%, respectively]. Similarly, at
this concentration (5 mM) sperm DNA damage, tail length and tail moment of cryopreserved
semen were significantly (P