The current study was carried out to investigate the improving roles of silymarin-plus® versus antibiotics on mare’s uterine environment quality (UEQ). Current study was carried on 23 mares aged between 7- 13y. Animals were subjected to clinical and gynecological examinations with detailed breeding history that revealed repeat breeding although there were apparently normal mares with normal breeding management. Animals were divided into three groups according to medications used in lavage; G1 (Silymarin-plus® ), G2 (Ciprofloxacin + Amikacin), and G3 (mixture between Silymarin plus® and Ciprofloxacin + Amikacin). Jugular blood samples were collected to assess the hormonal patterns (E2 & P4) in correlation with monitoring UEQ and follicular growth by ultrasonography. There was nonsignificant difference (P