A novel and highly selective kinetic spectrophotometric method was developed for the determination of cyclobenzaprine HCl. This method was based on monitoring the different kinetic characteristics of the reaction between the studied drug and potassium permanganate as an oxidizing agent in alkaline medium and the associated change in absorbance by time at λmax 605 nm. The experimental conditions such as the effects of KMnO4 concentration, amount of NaOH and sequence of addition were optimized. The stoichiometric ratio and the reaction mechanism between the drug and the oxidant were determined. Different kinetic methods such as the initial rate and the fixed time methods were utilized under the optimum conditions for construction the calibration curves for determination cyclobenzaprine HCl. Linear range of 2.0-10 μg mL-1 was established. The precision of the proposed method was satisfactory where the relative standard deviation (RSD) < 1.89 % and the error percent (Er %) was in range (-0.33-1.75 %). The proposed method was successfully applied for determination of cyclobenzaprine HCl in a dosage form. The results were compared with some reported methods.