This work was planned to study the effect of dog breeds on the physical and chemical properties of semen. A total number of 53 dogs of Caucasian (n=3), German shepherd (n=37), Malinois (n=8) and Rottweiler (n=5) breeds and mixed ages were used for semen collection along a year from 2016 to 2017. Semen was collected using an artificial vagina and examined for color, density, volume, pH, sperm individual motility, livability rate, cell concentration (SCC) and morphology. Seminal plasma was harvested after centrifugation and examined for testosterone hormone levels, aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP) and total antioxidant capacity. Results indicated that dog breeds markedly (p