Production of high quality embryos in vitro needs an efficient in vitro fertilization (IVF). Seminal origin is one of the important
factors that affects the success of in vitro embryo production. So our goal was to determine the effect of using fresh and frozen semen
in fertilization on developmental competence and cryo-survival of buffalo embryos. Buffalo oocytes were matured and fertilized in
vitro by fresh and frozen semen. After embryos evaluation, good quality morula and blastocysts were vitrified using 0.25 ml straws
and the post-warmed viability was assessed by further culture for 24 h. There was no significant difference in cleavage rate between
embryos derived from fresh and frozen semen, whereas the rate of embryo development to the morula (P