This study was carried out to estimate the haematological, serum biochemical and urological alterations associated with post parturient haemoglobinuria (PPH) on 70 haemoglobinuria and 30 apparently healthy buffaloes. Blood, serum and urine samples were collected and tested for various haematological, biochemical and urological parameters. Significant decrease in total erythrocyte count, Hb concentration, PCV %, lymphocyte%, serum inorganic phosphorus, total protein, albumin, glucose whereas significant increase in neutrophils%, serum urea, creatinine, total bilirubin and alkaline phosphatase. In PPH colour of urine in haemoglobinuria buffaloes ranged from red, dark red to coffee colour. Analysis of urine samples by Reagent urine strips showed positive for haemoglobin and protein. It was concluded that post parturient haemoglobinuria causes severe alterations in hematobiochemical and urological parameters in buffaloes.