Satellite navigation and communications system can substantially be disturbed by ionospheric perturbations. Consequently, monitoring ionospheric anomalous has great significance. In this study, we focus on the short-term irregular disturbances through a strong thunderstorm in Wuhan City, Hubei, China by using ground-based GNSS observations from dense Continuously Operating Reference Stations (CORS) with a sampling rate of 1s. The total electron content (TEC) was used to find possible perturbations after biases have been calibrated for the derived TEC. Additionally, the geomagnetic conditions and the state of solar radiation was checked in the study period to recognize the causes for the ionospheric disturbances. The maxima and minima values of TEC deviations were ~2.5 and 0.5 TECU, respectively. Three methods of Detrended Fluctuations Analysis (DFA) were applied to assess the ionospheric disturbances over GNSS CORS stations; “Multi-step numerical difference”(MSND), “6th order polynomials fitting” (PF), and “one-week average difference”(AD). The analyzed results showed that MSND has the lowest performance. Meanwhile, the fitted TEC data with 6th order polynomials technique presented an improvement and a discrepancy related to MSND. To resolve this discrepancy, we proposed AD technique, it accomplished the best performance related to the TEC disturbances and was compared with the other two techniques. The research findings showed that ionospheric disturbed electrons can be generated with various rates and different velocities through lightning influences.