| publication name | A quantitative PCR approach for quantification of functional genes involved in the degradation of polycyclic aromatic hydrocarbons in contaminated soils |
|---|---|
| Authors | Esmaeil Shahsavari,*, Arturo Aburto-Medina, Mohamed Taha, Andrew S. Ball |
| year | 2016 |
| keywords | |
| journal | MethodsX |
| volume | 3 |
| issue | Not Available |
| pages | 205-211 |
| publisher | Elsevier |
| Local/International | International |
| Paper Link | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4804383/ |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
Polycyclic aromatic hydrocarbons (PAHs) are major pollutants globally and due to their carcinogenic and mutagenic properties their clean-up is paramount. Bioremediation or using PAH degrading microorganisms (mainly bacteria) to degrade the pollutants represents cheap, effective methods. These PAH degraders harbor functional genes which help microorganisms use PAHs as source of food and energy. Most probable number (MPN) and plate counting methods are widely used for counting PAHs degraders; however, as culture based methodsonlycountasmall fraction(