DODECYL benzene sulphonic acid was used in preparation of a series of nonionic surfactants (II - V) a-c. The preparation was completed by the reaction of dodecyl benzene sulphonic acid with thionyl chloride followed by the alkylene diamine series (n = 2, 4, 6, 8) to give Ia-d, respectively. The later products (Ia –d) were reacted with ethylene oxide to produce nonionic surfactants (II - V) a-c. The structures of the prepared nonionic surfactants were confirmed by IR and 1HNMR spectra. The surface properties, biodegradability and biological activities of the prepared compounds were investigated. The obtained data show that these compounds have good surface and biological activities besides, reasonable biodegradability properties.