Sulfonated anionic surfactants were synthesized by the esterification of acrylic acid (AA) with different types of fatty alcohols (C16:0, C18:0, C18:1 and mixed Peach oil fatty alcohols) followed by addition of sodium bisulfite. The Peach oil was extracted from Peach kernel waste, its chemical characterization and fatty acids compositions were determined. The chemical structure of the prepared anionic surfactants was confirmed by FTIR and 1H NMR spectroscopic tools. The surface properties and thermodynamic parameters, which illustrate the spontaneity of the adsorption and micellization processes of the synthesized surfactants, were determined. Furthermore, the biological activity of these surfactants was examined against sulfate reducing bacteria (SRB) and showed the aggressive influence on their growth.