The effect of ducks growth hormone GH gene and total DNA on Japanese quails was studied. The level of GH was higher through ducks GH gene injection in air sac, embryo and combined effect than through DNA and control, but the increase level of GH was within the normal range of GH for birds. The determination of total protein percentage was higher through ducks GH gene effect than through ducks DNA and control. The results were stable from Ft to F3. The SDS-PAGE protein howed dditional ew f hree ands n the treated birds than control and these were stable from Ft to F3. The agarose gel electrophoresis of total DNA with restricted enzyme Eco RI showed a new band in the treated F,, F2, F3 and donor. In addition it showed a smeared Pattern intensity appeared on the gel in donor, treatments, F,, F2 and F3 than control and was stable from FI to F2 to F3.