The present study was conducted to examine the protective effect of olive leaf extract (OLE) against glycerol-induced oxidative
stress in rats. Sixty male albino rats were used and allocated randomly into four groups, each of 15 rats. Groups (1) and (2) were
administered intraperitoneally (i.p.) a single dose of 500 μL normal saline and hypertonic glycerol solution (10 mL/kg b.wt., 50%
v/v, in sterile saline), respectively, followed by a 24-h period of water deprivation. Group (3) was orally given OLE (500 mg/kg
b.wt.) for 22 days and glycerol as mentioned above on the 14th day of OLE administration followed by a 24-h period of water
deprivation. Group (4) was administered OLE alone. Five rats from each group were sacrificed and samples were collected 1, 5,
and 8 days after water deprivation. Alterations in hematobiochemical parameters, renal and hepatic oxidative stress markers, as
well as histopathology of the kidney and liver, were evaluated. Glycerol treatment resulted in significant hematological and
biochemical alterations as well as significant renal and hepatic oxidative stress. Administration of OLE has significantly ameliorated renal dysfunction, morphological alterations of kidney and liver, and relieved the oxidative stress. These findings show
obviously the role of oxidative stress and its relevance to renal dysfunction and suggest the ameliorative impact of OLE in
glycerol-induced acute kidney damage in rats, possibly due to its antioxidant properties.