【Background】
Since the malignancy of dog bladder cancer (BC) is higher than human BC, most BC bearing dogs die early. In our
laboratory, we established the method of bladder cancer organoid culture using the cancer stem cells in their urine
samples and demonstrated the organoids can be used for an anti-cancer drug sensitivity test. On the one hand, the
usage of traditional Chinese medicine has been increasing in veterinary medicine. Although not a few veterinarians
use traditional Chinese medicine for cancer, little is known about direct effects and detailed mechanisms of traditional
Chinese medicine on dog cancer cells and cancer stem cells.
【Object】
The purpose of this study is to clarify the effects of traditional Chinese medicines on BC bearing dogs by examining
the effects of several traditional Chinese medicines, traditional Chinese medical supplements and crude drug extracts
using a dog BC organoid culture system.
【Methods and Results】
Dog BC organoids were treated with 39 kinds of traditional Chinese medicines, traditional Chinese medical
supplements and crude drug extracts (10- 100 mg/ml) for 72 hours, respectively. The cell viability of the organoids
was assessed by Alamarblue assay. Among these drugs and supplements, treatment of shibe-ria and ro-ka suppressed
cell viability of dog BC organoids in a concentration-dependent manner.
【Discussion】
We for the first time demonstrated that traditional Chinese medical supplements, shiberia and roka had suppressive
effects of dog BC stem cells.