The disposition kinetics and serum availability of amikacin in broiler chickens after single intravenous (IV) and intramuscular (IM) administrations of 10 mg/kg body weight were investigated. The concentrations of the drug in the serum were measured using microbiological assay on samples collected at frequent intervals after drug administration. Following intravenous injection, the serum concentration-time curves were best described by a two compartment open model. The elimination half-life (t1/2β), volume of distribution at steady state (Vdss) and total body clearance (Cltot) of amikacin were 4.48 h, 501.03 ml/kg and 0.08 L/h/kg, respectively. After intramuscular injection of amikacin at the same dose the peak serum concentrations (Cmax) were 15.25 μg/ml and were obtained at 1.89 h (tmax), the elimination half-life (t1/2el) were 5.23 h and absorbtion half-life (t1/2ab) were 0.75 h. The systemic bioavailability was 95.20%. Amikacin was detected in liver and kidney for 5 days post a single IM injection and withdrawal time was 6 days.