This investigation aimed mainly to study the radioactive isotope of manganese (Mn–54) was used to study foliar absorption, translocation, distribution and consequently utilization of Mn in combination with soil application of Phosphorus on six-month- old mango seedlings (Hindi Bi-Sinara cv.). In this study, three rates of Mn (0.125, 0.25 and 0.50%) solutions were prepared using MnSo4 which foliar applied in combination with 4 rates of P (0.0, 25, 50 and 100 ppm) as soil application. Manganese solutions were labeled with Mn-54 carrier-free to give 250.000, 500.000 and 1000.000 cpm (count/min.) for Mn at 0.125, 0.25 and 0.50%, respectively. Mango leaves could absorb Mn-54 in appreciable activity and about 20% of it was upward and downward translocated. Approximately 60% of mobile Mn-54 was upward translocated and the rest of it (40%) was downward translocated. Moreover, utilization of Mn-54 by mango leaves ranged from 8-14% according to Mn rate. It means that spraying with MnSo4 is considered a good source for supplying mango seedlings with this element. Generally, not only can foliar application of Mn supply or correct Mn deficiency in mango plants within a few days, but it is also successful method for maximizing the efficiency of application and minimizing the accumulation of elements as pollutants in the soil.