Effective nursing collaboration behavior is essential for improving quality of services in health care system which has an effect on the nurses' quality of work life. The study aimed to assess head nurses' perception about collaboration and its relation to staff nurses' quality of work life. A descriptive correlational design was utilized to conduct this study. The study was conducted at inpatient Medical and Surgical departments. Two groups of subjects of this study: The first group; all head nurses (60). The second group; a convenient sample of (300) staff nurses and with at least two years of experience. Two tools were used for data collection: The first tool; Collaboration Behavior Scale, the second tool; Quality of Work Life Questionnaire. Results: Nearly half of head nurses had a high perception level about collaboration among staff nurses, while more than one third of them had a moderate perception level. Nearly half of staff nurses had a high level of perception of total quality of work life, while, more than one quarter of them had a moderate level of perception. Conclusion: There was a statistical significant positive correlation between head nurses' perception about collaboration and staff nurses' perception about quality of work life. The study recommended: Staff development program is need for nurses managers to integrate staff nurses in decision-making and to deal effectively with new ideas to promote satisfactory organizational climate. Hospital manager should paying attention to the financial aspect (salary) of staff nurses to meet life needs.