The Taguchi design has been used to optimize citrine factors affecting on specific processes. Lactobacillus
paracasei ATCC 25,302 was used for cholesterol assimilation and applied the experimental factorial
design to optimize cholesterol assimilation conditions. The model predicted that the optimal conditions
for cholesterol assimilation were 120 lg /ml, incubation time 96 h, bile salts 0.1 %, probiotic dose 200 ll
and initial pH 6.5. The cholesterol assimilation increased as the cholesterol concentration increased up to
120 lg /ml, at which the maximum cholesterol assimilation (93%).

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