Staff Members - Benha University
Biological, serological and molecular identification of Xathomonas sp antibiotic resistance infecting fruit and vegetative crops
Middle East Journal of Applied Sciences • 2021
Publication Information
Authors
1Fawizia M. A. Abnsaira, 3Attia A. A., 3Abeer A. Khattab, 3Mervat G. Hassen, 3Amer
M.M. and 2El Dougdoug K.A.
Keywords
ABSTRACT
Antibiotics have been used for the control of plant pathogenic bacteria and it has become a
problem in pathosystems. The current study aimed to detection plasmid curing in species of the genus
Xathomonas antibiotic resistance isolated from citrus, almonds and beans. Methods: Common
bacteria blight disease in citrus , almond and beans were detected by external symptoms and DASELISA
.The casual against was detected and isolated on selected media by morphological and
biochemical characteristics of the colonies, Indirect immunofluorescence assay, bioassay of leaf
detached leaves and Hgene by PCR .The isolated bacteria were tested antibiotic sensitivity.
Results: Typical symptoms of common blight disease Xanthomonas sp on citrus, almond and
beans that, necrotic spots surrounded by chlorotic haloes, sunken and corky lesions with oozing gum.
Bright yellow spots on the underside of leaves, followed by erumpent brownish lesions. Symptoms
were recorded at late summer through to autumn 2017. The canker, chlorotic haloes and halo blight
spots gave positive serological reaction with polyclonal antibodies specific Xanthomonas sp by DASELISA
. Xanthomonas sp was isolated on YPGA medium and yellow pigment colonies from naturally
infected citrus, almond and beans leaves. Xanthomonas sp isolates were identified which showed
some differences of physiological and biochemical properties. Xanthomonas isolates inoculation of
intact .immature leaves of Nicotiana tabacum cv samson showed 65–75% to fully expanded were
preferred and the lesions develop 15–20 days. Three Xanthomonas Hgene sequence X.campestris
pv. campestris str. ATCC 33913. Phylogenetically sequence comparison confirmed that
Xanthomonas spp isolates closest Hgene genetic homologues all belong to X. citri subsp. citri, X.
arboricola and X. campestris pv. phaseoli. Antibiotics sensitivity of Xanthomonas sp isolates showed
resistance against Penicillin G, Streptomycin and more resistance against, Sulphadiazole and
Tetracycline. On the contrary Xanthomonas isolates after plasmids curing treatment showed
sensitivity where formed inhibition zone with different area to Penicillin G, Streptomycin,
Sulphadiazole and Tetracycline.
Journal
Middle East Journal of Applied Sciences
Publisher
Not Available
Volume
8
Issue
4
Pages
1512-1525
publication.type
Local
Paper Link
Open Link
Supplementary Materials
Not Available
Abstract
ABSTRACT
Antibiotics have been used for the control of plant pathogenic bacteria and it has become a
problem in pathosystems. The current study aimed to detection plasmid curing in species of the genus
Xathomonas antibiotic resistance isolated from citrus, almonds and beans. Methods: Common
bacteria blight disease in citrus , almond and beans were detected by external symptoms and DASELISA
.The casual against was detected and isolated on selected media by morphological and
biochemical characteristics of the colonies, Indirect immunofluorescence assay, bioassay of leaf
detached leaves and Hgene by PCR .The isolated bacteria were tested antibiotic sensitivity.
Results: Typical symptoms of common blight disease Xanthomonas sp on citrus, almond and
beans that, necrotic spots surrounded by chlorotic haloes, sunken and corky lesions with oozing gum.
Bright yellow spots on the underside of leaves, followed by erumpent brownish lesions. Symptoms
were recorded at late summer through to autumn 2017. The canker, chlorotic haloes and halo blight
spots gave positive serological reaction with polyclonal antibodies specific Xanthomonas sp by DASELISA
. Xanthomonas sp was isolated on YPGA medium and yellow pigment colonies from naturally
infected citrus, almond and beans leaves. Xanthomonas sp isolates were identified which showed
some differences of physiological and biochemical properties. Xanthomonas isolates inoculation of
intact .immature leaves of Nicotiana tabacum cv samson showed 65–75% to fully expanded were
preferred and the lesions develop 15–20 days. Three Xanthomonas sp isolates gave positive
serological reaction with polyclonal antibodies specific Xanthomonas sp by DAS-ELISA. The Hgene
sequence in the genome of Xanthomonas spp isolates suggested the original assignment of this
isolates to the species X.citri, X.arboricola and X. campestris based on Hgene sequence X.campestris
pv. campestris str. ATCC 33913. Phylogenetically sequence comparison confirmed that
Xanthomonas spp isolates closest Hgene genetic homologues all belong to X. citri subsp. citri, X.
arboricola and X. campestris pv. phaseoli. Antibiotics sensitivity of Xanthomonas sp isolates showed
resistance against Penicillin G, Streptomycin and more resistance against, Sulphadiazole and
Tetracycline. On the contrary Xanthomonas isolates after plasmids curing treatment showed
sensitivity where formed inhibition zone with different area to Penicillin G, Streptomycin,
Sulphadiazole and Tetracycline.
Antibiotics have been used for the control of plant pathogenic bacteria and it has become a
problem in pathosystems. The current study aimed to detection plasmid curing in species of the genus
Xathomonas antibiotic resistance isolated from citrus, almonds and beans. Methods: Common
bacteria blight disease in citrus , almond and beans were detected by external symptoms and DASELISA
.The casual against was detected and isolated on selected media by morphological and
biochemical characteristics of the colonies, Indirect immunofluorescence assay, bioassay of leaf
detached leaves and Hgene by PCR .The isolated bacteria were tested antibiotic sensitivity.
Results: Typical symptoms of common blight disease Xanthomonas sp on citrus, almond and
beans that, necrotic spots surrounded by chlorotic haloes, sunken and corky lesions with oozing gum.
Bright yellow spots on the underside of leaves, followed by erumpent brownish lesions. Symptoms
were recorded at late summer through to autumn 2017. The canker, chlorotic haloes and halo blight
spots gave positive serological reaction with polyclonal antibodies specific Xanthomonas sp by DASELISA
. Xanthomonas sp was isolated on YPGA medium and yellow pigment colonies from naturally
infected citrus, almond and beans leaves. Xanthomonas sp isolates were identified which showed
some differences of physiological and biochemical properties. Xanthomonas isolates inoculation of
intact .immature leaves of Nicotiana tabacum cv samson showed 65–75% to fully expanded were
preferred and the lesions develop 15–20 days. Three Xanthomonas sp isolates gave positive
serological reaction with polyclonal antibodies specific Xanthomonas sp by DAS-ELISA. The Hgene
sequence in the genome of Xanthomonas spp isolates suggested the original assignment of this
isolates to the species X.citri, X.arboricola and X. campestris based on Hgene sequence X.campestris
pv. campestris str. ATCC 33913. Phylogenetically sequence comparison confirmed that
Xanthomonas spp isolates closest Hgene genetic homologues all belong to X. citri subsp. citri, X.
arboricola and X. campestris pv. phaseoli. Antibiotics sensitivity of Xanthomonas sp isolates showed
resistance against Penicillin G, Streptomycin and more resistance against, Sulphadiazole and
Tetracycline. On the contrary Xanthomonas isolates after plasmids curing treatment showed
sensitivity where formed inhibition zone with different area to Penicillin G, Streptomycin,
Sulphadiazole and Tetracycline.