Staff Members - Benha University
P ARTIAL BL V RNAs ARE PRESENT IN PLASMA EXOSOMES OF BL V INFECTED ANIMALS
Ruminant science • 2016
Publication Information
Authors
Marawan A Marawan
1,2,*
, Abdel-moneim M Moustafa
1
, Faysal K Arnaout
1
and El Sayed M Galila
Keywords
Exosomes, BL V , R T -PCR, sequence analysis, Western-blot
Journal
Ruminant science
Publisher
Not Available
Volume
5
Issue
2
Pages
133-142
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Exosomes are membranous nano-vesicles of endocytic origin that are released by almost all cell types and play an
important role in cell-to-cell communication. Many researches have been carried out to illustrate the role of exosomes in
the life cycle and pathogenesis of RNA viruses, however , information regarding their role in Bovine leukemia virus (BL V)
is meagre. Herein we tried to investigate the role of exosomes isolated from plasma in BL V life cycle. Exosomal fractions
were isolated from 18 fresh BL V-positive plasma samples by size exclusion chromatography (SEC) and then were tested
using western blotting, RT -PCR and sequence analysis.Our results showed that absence of BL V enveloped protein (env)
in all tested exosomal fractions, on the other hand around 22% of samples showed the presence of partial BL V RNA in
their exosomes. From this approach, it was emphasized that the exosomes may incriminate in BL V pathogenesis through
transfer of viral RNA from infected to uninfected B cells.
important role in cell-to-cell communication. Many researches have been carried out to illustrate the role of exosomes in
the life cycle and pathogenesis of RNA viruses, however , information regarding their role in Bovine leukemia virus (BL V)
is meagre. Herein we tried to investigate the role of exosomes isolated from plasma in BL V life cycle. Exosomal fractions
were isolated from 18 fresh BL V-positive plasma samples by size exclusion chromatography (SEC) and then were tested
using western blotting, RT -PCR and sequence analysis.Our results showed that absence of BL V enveloped protein (env)
in all tested exosomal fractions, on the other hand around 22% of samples showed the presence of partial BL V RNA in
their exosomes. From this approach, it was emphasized that the exosomes may incriminate in BL V pathogenesis through
transfer of viral RNA from infected to uninfected B cells.