| publication name | Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity inStevia rebaudiana (Bertoni). |
|---|---|
| Authors | Elham Shehab, M. M. M. Bekhit, M. S. Abdel-Sabour, T. A. M. El-aqaad and and A. A. El-Sheikh |
| year | 2018 |
| keywords | chromosomes, Stevia, bivalents, SDS-PAG Eelectrophoresis, ISSR |
| journal | 4th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada , 4-7 April 2018 , Egypt |
| volume | 1 |
| issue | 1 |
| pages | 177-186 |
| publisher | Faculty of Agriculture, Moshtohor |
| Local/International | International |
| Paper Link | Not Available |
| Full paper | download |
| Supplementary materials | Not Available |
Abstract
Chromosome number was investigated in a number of strains of Stevia rebaudiana. The chromosomes were analyzed during mitosis and different stages of meiosis. All stevia strains were found to had 2n = 22. The number of bivalents at diakinesis was 11 for all the studied strains. Analysis of SDS-PAGE of soluble protein revealed complete similarity (100%) among the six strains of Stevia while two strains (broad and narrow leaves) are different in one band. Eight genotypes were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. The level of polymorphism detected among the eight strains of stevia was 100.00% using inter simple sequence repeat (ISSR). A total of 231 clear bands were generated, out of which 34 (14.72 %) were unique bands. The total number of markers varied from 1 to 12 with a mean of 5.67 markers per primer.