Staff Members - Benha University
Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity inStevia rebaudiana (Bertoni).
4th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada , 4-7 April 2018 , Egypt • 2018
Publication Information
Authors
Elham Shehab, M. M. M. Bekhit, M. S. Abdel-Sabour, T. A. M. El-aqaad and and A. A. El-Sheikh
Keywords
chromosomes, Stevia, bivalents, SDS-PAG Eelectrophoresis, ISSR
Journal
4th International Conference on Biotechnology Applications in Agriculture (ICBAA), Benha University, Moshtohor and Hurghada , 4-7 April 2018 , Egypt
Publisher
Faculty of Agriculture, Moshtohor
Volume
1
Issue
1
Pages
177-186
publication.type
International
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Chromosome number was investigated in a number of strains of Stevia rebaudiana. The chromosomes were analyzed during mitosis and different stages of meiosis. All stevia strains were found to had 2n = 22. The number of bivalents at diakinesis was 11 for all the studied strains. Analysis of SDS-PAGE of soluble protein revealed complete similarity (100%) among the six strains of Stevia while two strains (broad and narrow leaves) are different in one band. Eight genotypes were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships. The level of polymorphism detected among the eight strains of stevia was 100.00% using inter simple sequence repeat (ISSR). A total of 231 clear bands were generated, out of which 34 (14.72 %) were unique bands. The total number of markers varied from 1 to 12 with a mean of 5.67 markers per primer.