Staff Members - Benha University
Using Antibiotic Kanamycien and Estenrase Isozyme as Efficient methods for Detecting the Transformed DNA in Tomato interspecific hybridization.
The Egyptian Journal of Applied science (EJAS) • 1997
Publication Information
Authors
Bekhit, M. M. M.;Abdel-Sabour, M. S.; El-Said, M. Fareda; Nazeem, H. R.; Hassan, A. M.; Mageid, A. A. and Refaat, M. H.
Keywords
Not Available
Journal
The Egyptian Journal of Applied science (EJAS)
Publisher
Egyptian Society of Applied Science in collaboration with Zagazig University.
Volume
12
Issue
No. 9
Pages
141-154
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
Higher percentages of survival calli were detected after transformation of the wild type DNA (LA: 385) on selective medium with 1000 mg/L kanamycien for two, three, four and five weeks in the three cultivated varieties; UC-97/3, Peto-86 and Castle Rock (treated and untreated with DNA) beside the wild type parent. For using esttease isozyme as a marker for success of interspecific hybridization.it was revealed that only a band at 0.5 was transferred to the transformed clones with DNA from wild parent to ensure transformation to the clones of parents.