This study was conducted to demonstrate the effect of castration on the structure of vesicular glands of the Egyptian Nubian (Zaraibi) goat. Vesicular glands of castrated (n = 4) and intact (n = 6) goat were used for histological and immunohistochemical evaluations. In this study, we report the difference in cell specific expression of androgen receptor (AR) and cyclooxygenase-2 (COX-2) in the vesicular glands of castrated and intact goats. In both castrated and intact goats, the present study revealed no immunopositive cells for AR or COX-2 in the fibromuscular stroma meanwhile, AR and COX-2 containing immunoreactive cells were restricted only to the epithelium of the secretory acini of the vesicular gland. Such finding suggests androgen and COX-2 as important regulators for the growth and secretory activity of epithelial cells in the vesicular gland of goats. Overall, the vesicular gland of castrated goats showed significantly (P < 0.05) lower AR and COX-2 immuno-expression than intact goats indicating that both AR and COX-2 are androgen dependent