Staff Members - Benha University
Cloning and Nucleotide Sequence of 16S-like Rna Coding from Lipolytic Pseudomonas Fluorescens Eg Strain from Animal Blood Weste
• 2014
Publication Information
Authors
Hazaa, M.M. Motwely, M.R. and Hassan, R.A.
Botany Department, Faculty of Science, Benha University, Egypt
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publication.type
International
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Supplementary Materials
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Abstract
An lipolytic producing bacterial strain was isolated from slaughter house wastes (animal blood).
In order to identify the bacterial strain microbiological, physiological and biochemical tests were
performed, along with 16S-like rRNA and DNA-DNA hybridization analysis. The bacterium was found
to be a strain of Pseudoonas fluorescens which was designated as (EG). The strain was grown in SHBM
medium. Ps. Fluorescens EG strain secretes a thermostable lipase into the extracellular medium. To further
characterize the strain phy logenetic analysis of the strain EG were performed using 16S rRNA anaysis. The
1451 bp 16S rRNA gene was amplified from EG strain and sequence compared with that of other
Pseudomonas spp. Which showed up to 8 8% to Ps. Fluorescens strains (chloro, coronas; congs. S, syrin,
cari, Flo. Se, Flo.S and Flo.S). The phy logenetic tree developed from this comparative study also indicated
that EG was closer to Ps. fluorescens with an aberration to Ps. fluorescens Strains EG. Nucleotide sequence
of 16S-rRNA was recorded in gene bank with Accession number EU 2703406.
In order to identify the bacterial strain microbiological, physiological and biochemical tests were
performed, along with 16S-like rRNA and DNA-DNA hybridization analysis. The bacterium was found
to be a strain of Pseudoonas fluorescens which was designated as (EG). The strain was grown in SHBM
medium. Ps. Fluorescens EG strain secretes a thermostable lipase into the extracellular medium. To further
characterize the strain phy logenetic analysis of the strain EG were performed using 16S rRNA anaysis. The
1451 bp 16S rRNA gene was amplified from EG strain and sequence compared with that of other
Pseudomonas spp. Which showed up to 8 8% to Ps. Fluorescens strains (chloro, coronas; congs. S, syrin,
cari, Flo. Se, Flo.S and Flo.S). The phy logenetic tree developed from this comparative study also indicated
that EG was closer to Ps. fluorescens with an aberration to Ps. fluorescens Strains EG. Nucleotide sequence
of 16S-rRNA was recorded in gene bank with Accession number EU 2703406.