Staff Members - Benha University
MOLECULAR IDENTIFICATION OF SOME ISOLATES OF PSEUDOMONAS AERUGINOSA ISOLATED FROM PROCESSED FOODS
• 2012
Publication Information
Authors
Dalia, A.A.Moustafa*; MJVL Hazaa*; Kh.A. El-Doug
doug **; Nahed M. Ayaat* and Abeer A. Khattab* *Botany Department, Faculty of
Science ,Benha university, Benha, Egypt. **Department of Microbiology, Faculty of
Agriculture Ain Shams University Key Words:
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Local
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Abstract
The identification of 16SrRNA structural gene of four Ps .aeruginosa isolates
contaminated processd foods was done by PCR and sequencing of amplicons. Genomic
DNA isolated from Ps.aeruginosa isolates namely (Ps l,Ps 2,Ps 3,Ps 4)using a
lysozyme dodecyl sulfate lysis procedures with high quality and substantly free DNA
contamination. The DNA was then used as a template for PCR to amplify the 16srRNA
gene via the QLAGEN PCR system by the use of oligo (dt),fGl and rP2 primer sets,
partially length of 16srRNA gene could be synthesized . The amplified 16srRNA gene was
used as a tempelate using the internal primer combination (fGl and rP2) in PCR to confirm
it's specificity to the Ps aeruginosa 16srRNA gene as a PCR product with a size of
about 371 bp DNA was amplified of four Ps . aeruginosa isolates.
contaminated processd foods was done by PCR and sequencing of amplicons. Genomic
DNA isolated from Ps.aeruginosa isolates namely (Ps l,Ps 2,Ps 3,Ps 4)using a
lysozyme dodecyl sulfate lysis procedures with high quality and substantly free DNA
contamination. The DNA was then used as a template for PCR to amplify the 16srRNA
gene via the QLAGEN PCR system by the use of oligo (dt),fGl and rP2 primer sets,
partially length of 16srRNA gene could be synthesized . The amplified 16srRNA gene was
used as a tempelate using the internal primer combination (fGl and rP2) in PCR to confirm
it's specificity to the Ps aeruginosa 16srRNA gene as a PCR product with a size of
about 371 bp DNA was amplified of four Ps . aeruginosa isolates.