Three experiments were conducted to evaluate potential of using zengebare officinale extract as treatment to fresh water fishes from bacterial diseases. Also the use of ethanol-extracted from the medicinal plant, Zingebare officinale as a growth and immunity promoter for Oreochromis niloticus (L.) fingerlings. Fish (Average 12.33 g) were randomly distributed into four treatments; three replicates each at a rate of 15 fish per 140- L aquarium. Fish were fed one of the tested diets containing 0.0. 0.5 %. 1.0 %, or 1.5 % Zingebare officinale extract for 10 weeks. After the feeding trial, fish of each treatment were challenged by pathogenic Pseudomonas aeruginosa and Pseudomonas flourscence. and they were kept under observation for 10 days to follow up any abnormal clinical signs and the daily mortality rate. The growthpromoting influence of Zingebare officinale extract was observed on fish. The results showed that the maximum growth was observed at 1 % Zingebare officinale extract as compared to the control. No significant differences in fish survival were reported among the experienced treatments at (P>0.05). falling within the range of 93.3 100%. total protein, albumin, and globulin increased significantly (P