The main objective of this contribution is to introduce a novel multi-tag anti-collision protocol based on Look-Up-Table (LUT) scheme. The proposed protocol includes an enhancement for the spectrum utilization and coding capacity. This is accomplished by transferring the tag-ID to be stored in a table in the main memory of the reader (look-up-table). Moreover, the unique signature of each tag represents the address of the tag's ID. The proposed protocol is modeled and simulated for identifying 10-chipless tags in order to set the regulations of the tag and reader design. Moreover, a novel real-world testbed for the multi-tag UWB chipless RFID system based on a software defined radio is introduced. In this testbed, all the signaling schemes related to the transmitted signal, detection techniques, empty room calibration for the clutter removal process and identification protocol are applied.