Staff Members - Benha University
Establishment of regeneration and transformation systems of F114 sugarcane cultivar. Pak. J. Biotechnol., 2(1-2): 24-35.
• 2005
Publication Information
Authors
Attia, O.A.; Mahdy, A.M.M.; Hafez, M.A.; Sadik, A.S. and Naglaa A. Abdu-Allah
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publication.type
Local
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Abstract
Sugarcane is one of the most important (two sugar) crops for sugar production in Egypt. In this study, an experiment was conducted to establishment the regeneration system for sugarcane., F144 using leaf bases as a explants through somatic embryogenesis. The effect of 2,4-D, NAA, IAA and kinetin hormones on the sugarcane regeneration was studied. The microprojectile mediated method was used to transform plasmid pAB6 carrying the gus and bar genes as reporter and selectable markers respectively. The presence of the trans- genes was confirmed by PCR analysis through which amplified fragments with sizes of about 1800(gus) and 540 bp (bar) were detected in transgenics. GUS assay and leaf painting detect the expression of both gus and bar genes.
Key words: Sugarcane, microprojectile mediated method, plasmid pAB6, GUS and BAR genes.
Key words: Sugarcane, microprojectile mediated method, plasmid pAB6, GUS and BAR genes.