Nursing is the profession that based on collaborative relations that concentrate on high-quality care. Interpersonal conflict in the nursing profession is inevitable and can lead to nurses' job dissatisfaction with low-quality patient care. Aim: the study objective was conducted to assess the level of job satisfaction, interpersonal conflict and to ascertain the relationship between nurses' job satisfaction and interpersonal conflict among nurses in intensive care units at Benha University hospital.Methodology: research design used for the study was a cross-sectional correlational design with three months of monitoring. The study conducted in all available nurses working in critical care units at Benha University hospital in Benha city, Egypt which affiliated to the Ministry of health. After seeing the inclusion and exclusion criteria, the study sample was 150 professional nurses (150 out of 216) with a minimum of one year of experience; and willing to participate in the study. The study made use of a three-part researcher-made questionnaire, developed from the literature review. A convenient type of sample was used to collect the data. The researchers designed an interviewing assessment sheet, and the interpersonal conflict questionnaire and Bar- job satisfaction questionnaire are used to collect the data. Results: The findings showed that; the large percentages of the studied sample (78%) have interpersonal conflict. Majority of subjects were dissatisfied with their work (82.7%), and there was a significant negative correlation between interpersonal conflict and job satisfaction among studied nurses. Conclusions: interpersonal conflict negatively correlates with job satisfaction, and there were significant differences among studied nurses regarding their socio-demographic characteristics concerning interpersonal conflict and job satisfaction level except for sex which showed no significant relation.