This experiment aimed to evaluate the effect of dietary supplementation of L-carnitine and amino acids (methionine+lysine) for Nile tilapia (Oreochromis niloticus), the basal diet (300 g protein and 19 MJ Gross energy kg-1 dry matter) was formulated and served as control (D1). The based diet was supplemented with 300 mg L-carnitine (D2), 5g methionine+10g lysine (D3), 7g methionine+15g lysine (D4), 5g methionine+10g lysine+300 mg L-carnitine (D5), or 7g methionine+15g lysine+300 mg L-carnitine (D6). A total number of 300 apparent healthy Nile tilapia fry (1.63±0.07g) were randomly distributed into 18 rectangular aquaria (160 liter for each) and each aquarium holding 25 fry was then randomly assigned to one of three replicates of six diets. Fish group fed D6 retained the highest significant body weight (BW), body length (BL), weight gain (WG), specific growth rate (SGR) and significantly (P