The present study was carried out in order to investigate the response of Nile tilapia fry to some commercial probiotics (Biogen, Megalo and yeast) and some spices oils (garlic and fennel oils) and the combinations of Megalo and each of garlic and fennel as dietary feed additives. Therefore, 8 diets were prepared, the 1st diet (without any supplementation) as control (D1) and the others were supplemented by 0.2% Biogen (D2), 0.3% yeast (D3), 0.2% Megalo (D4), 0.1% Garilc oil (D5), 0.1% fennel oil (D6), 0.1% garlic oil+0.2% Megalo (D7) and 0.1% fennel oil+0.2% Megalo (D8). The feeding trial lasted for 90 days. At the termination of the experiment, D1(control diet) released the lower body weight (12.15 g) compared to 20.80, 21.45, 21.40, 20.50, 20.72, 21.95 and 19.56 g for the different experimental diets D2, D3, D4, D5, D6, D7 and D8, respectively, and the differences between body weight for the control group (D1) and each of the other treatments were significant (P0.05) affected hematocrite (Ht) percentages and liver transferase enzymes (AST and ALT) values while hemoglobin (Hb) level showed some variations. Probiotics or spices oils had no significant effect on proximate analysis of Nile tilapia.