Blood transfusion for children is considered an integral part of everyday life in an acute hospital setting and is a matter of life or death. Therefore, nurses should have a competent and safe transfusion practice. Aim: The present study aimed to evaluate the effect of training program on nurses' competent practice towards children receiving blood transfusion. Design: A quasi-experimental design was utilized to conduct this study. Settings: The study was carried out in Pediatric Medicine departments at Benha University Hospital, at Benha City. Sample: A convenient sample of 60 nurses from the previously mentioned settings. Tools: Tools of data collection included; Tool (I): A structured interviewing questionnaire sheet to explore nurses’ personal data and their knowledge regarding blood transfusion for children to explore nurses’ personal data and their knowledge regarding blood transfusion for children,,Tool (II): Observational checklist to assess the actual nurses' practices towards caring for children receiving blood transfusion. Results: The present study results revealed that, the mean age of nurses was 27.97±5.92 years and more than half of them weren’t attending training courses related to blood transfusion. There were highly statistical significant differences (P