Coronavirus 2019 is one of the major pathogens that was and still one of the most serious global health threats. Aim of study: Was to evaluate the effect of educational guidelines on nursing Students’ knowledge and practice regarding limitation the spread of COVID- 19. Research design: Quasi-experimental design was utilized to conduct the study. Setting: The study was conducted at Faculty of Nursing, Benha University. Subject: A stratified random sample of 241 undergraduate students from Faculty of Nursing, Benha University. Data collection tools: A Structured Assessment Questionnaire was used, composed of three parts; (1) Students' personal data and medical history (2) Students’ Knowledge Assessment about COVID-19. (3) Students’ Practices Assessment. Results: 92.5% of the nursing students had satisfactory level of knowledge post the educational guidelines compared to 12.8% pre the intervention and there were significant improvement in mean scores of their practices of preventive measures to limit the spread of COVID-19. There was statistical significant positive correlation between their total level of knowledge and practices score regarding COVID-19. Conclusion: The educational guidelines effectively improved nursing students' knowledge and practices regarding limitation the spread of COVID-19. Recommendations: Further educational programs are needed for nursing students regarding limitation the spread of COVID-19 on large sample selected from all nursing faculties in Egypt.