This study aimed to investigate the effect of hypomagnesemia on glutathione redox cycle, Nitric oxide, serum cortisone and insulin. Twelve healthy Baladi male goats were used in this study. They were divided into 2 groups; the first one contained 5 animals and kept as control group. The other group contained 7 animals subjected to experimental hypomagnesemia by daily oral administration of potassium chloride and citric acid until development of the characteristic signs of hypomagnesemia. Blood samples were collected at 6, 12, 18 and 24 days of administration .The recorded data revealed that serum magnesium concentration decreased in experimental group compared with the values of control group. There was a significant decrease in serum nitrate, and glutathione peroxidase (GSH-Px), glutathione (GSH), glutathione reductase) GR-ase), glutathione–S- transferase (GST) and total superoxide dismutase) t-SOD) activities, whereas catalase) CAT) activity was significantly increased in hypomagnesemic group compared to the control group. Serum insulin levels decreased significantly, whereas serum cortisone levels increased in comparison to the values in the control healthy group. From these results we can conclude that nitric oxide release, glutathione redox cycle activities, insulin and cortisone levels were markedly affected by hypomagnesemia. These changes may be the main causes of tissue injury and vascular changes that usually accompanying hypomagnesemia.