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Accumulation of a 70 kDa stress protein in the Nile Tilapia, Oreochromis niloticus, and its use as a biomarker of Cu exposure.
Egyptian Journal of Aquatic Boilogy & Fisheries • 2006
Publication Information
Authors
Sharaf-Eldeen, K.
Keywords
Hsp70, fish, copper, Nile Tilapia, biomarker
Journal
Egyptian Journal of Aquatic Boilogy & Fisheries
Publisher
Ain Shams University
Volume
10
Issue
2
Pages
19-31
publication.type
Local
Paper Link
Not Available
Supplementary Materials
Not Available
Abstract
The induction of 70 kDa heat - shock protein (hsp70) in the Nile
tilapia, Oreochromis niloticus, was investigated. The accumulation ofhsp70 was determined in tissues of the liver, gills, spleen and heart of heat shocked fish. Other specimens were also exposed for 96 hr to four concentrations (1,0, 0.5, 0.1 and 0.001 mg/1) of Cu as copper sulphate and the accumulation of hsp70 was determined in the liver and gills. In both experiments, antibodies raised against hsp70 were employed as probes for dot-blot analysis and immunobinding assay of tilapia tissues. Decreasingly, hsp70 was abundant in the spleen, gills, heart and iiver of heat shocked fish. Also, data showed greater hsp70 level in the liver and gills of fish exposed to elevated Cu concentrations than the that of controls.
These data suggest that hsp70 accumulation may hold a promise
as a molecular indicator of contaminant exposure and may give an early warning of adverse biological effects. The present study indicates that the biomarker method is very easy to use, practical, rapid, and sensitive for assessing water quality in tropical water and is recommended for their incorporation into the future monitoring program.
tilapia, Oreochromis niloticus, was investigated. The accumulation ofhsp70 was determined in tissues of the liver, gills, spleen and heart of heat shocked fish. Other specimens were also exposed for 96 hr to four concentrations (1,0, 0.5, 0.1 and 0.001 mg/1) of Cu as copper sulphate and the accumulation of hsp70 was determined in the liver and gills. In both experiments, antibodies raised against hsp70 were employed as probes for dot-blot analysis and immunobinding assay of tilapia tissues. Decreasingly, hsp70 was abundant in the spleen, gills, heart and iiver of heat shocked fish. Also, data showed greater hsp70 level in the liver and gills of fish exposed to elevated Cu concentrations than the that of controls.
These data suggest that hsp70 accumulation may hold a promise
as a molecular indicator of contaminant exposure and may give an early warning of adverse biological effects. The present study indicates that the biomarker method is very easy to use, practical, rapid, and sensitive for assessing water quality in tropical water and is recommended for their incorporation into the future monitoring program.